FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979770
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39690.20047783328366428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61350.036.2196661
GGTATCA29750.031.4370351
TATCAAC70500.031.3021772
TCAACGC74250.029.7288574
AACGCAG73950.029.7120196
CAACGCA74650.029.5246735
ATCAACG75600.029.257823
GTGGTAT13650.027.5066721
ACGCAGA79750.027.4229987
AGAGTAC78850.027.26353611
CGCAGAG82850.026.561388
CAGAGTA82350.026.22691510
GCAGAGT86400.025.4700269
TGGTATC14900.024.7227572
AGTACGG63150.023.58154713
GAGTACG63550.023.53857812
GAGTACT32000.020.44478612
GTACGGG74600.019.73372714
AGTACTT34200.019.13107313
TACGGGG34100.019.08820715
Produced by FastQC (version 0.11.5)