FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453555
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG119590.8227414855303032No Hit
GACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCACTA84070.5783750872859988No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCACTAACACTATCCCACAC83140.5719769805752104No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC75880.5220304701232495No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGG63410.4362408027216032No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG60370.415326561430424No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT54410.3743236410042964No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC48900.336416578664034No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTC46040.31674068060720095No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT44700.3075219031959575No Hit
CACTAACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGTGG44070.303187701875746No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG42460.2921114096129834No Hit
GTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC42240.2905978789932269No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41330.2843373659751437No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40830.2808975236575155No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40800.28069113311845784No Hit
GTGTTAGTGATCATTGGATGTTCGGCGGAGGGACCGACCTGACCGTCCTA40420.27807685295706047No Hit
GATTATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGG39520.2718851367853298No Hit
ACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCACTAA39450.2714035588608618No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38490.26479906161101574No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATT38290.2634231246839645No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35790.2462239130958237No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG35190.2420961023146699No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG34700.23872505684339432No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC34620.2381746820725738No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACT33420.2299190605102662No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG32800.22565365603640727No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG32500.2235897506458304No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT32370.22269539164324706No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32250.22186982948701633No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG31820.2189115650938561No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGC31690.21801720609127279No Hit
GTGTGGGATAGTGTTAGTGATCATTGGATGTTCGGCGGAGGGACCGACCTGACCGTCCTAGGTCAGCCCAAGG31430.21622848808610612No Hit
GGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC31230.21485255115905486No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA30360.20886722552638184No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30320.2085920381409716No Hit
GGTGATAATCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT30250.2081104602165037No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29920.2058401642868691No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28840.19841010488079228No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCA28690.19737815218550384No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGTGG27970.19242477924811927No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26840.18465073561027961No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT26710.1837563766076963No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA26610.18306840814417066No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT26360.18134848698535658No Hit
CGATTACCTGTGGGGGAAACAACATTGGAATTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC25690.17673909827973486No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25680.17667030143338228No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG24760.17034099156894647No Hit
CCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACA24710.16999700733718368No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24370.1676579145611965No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24180.16635077448049782No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA24060.16552521232426703No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAAACAA23860.1641492753972158No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23810.16380529116545298No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23800.16373649431910042No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATTCCAATGTTGTTTCCCCCACAGGTAATCGTGGC23590.16229176054569658No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAATTAAAAGTGTG23290.16022785515511967No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGG22910.15761357499372228No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC22900.15754477814736972No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22590.15541207591044026No Hit
CATCATAGACGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG21990.15128426512928647No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGT21680.14915156289235704No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21480.14777562596530575No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21460.14763803227260064No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20750.14275345618156865No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20670.1422030814107481No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20410.1404143634055815No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC20310.13972639494205585No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20210.13903842647853024No Hit
CTTTTAATTCCAATGTTGTTTCCCCCACAGGTAATCGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG20120.13841925486135714No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT19980.13745609901242128No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19850.13656174000983795No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19790.13614895893172257No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCA19620.134979412543729No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19590.1347730220046713No Hit
ACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC19230.13229633553597903No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18620.12809972790847268No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18600.12796213421576755No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC18460.12699897836683166No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG18380.12644860359601115No Hit
AGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCC18360.12631100990330604No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG18320.12603582251789577No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18240.12548544774707526No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18130.12472868243719709No Hit
GCTGGTACCAGTGCACACTTTTAATTCCAATGTTGTTTCCCCCACAGGTAATCGTGGCCGTCTTTCCTGGGGCCA18090.12445349505178682No Hit
CACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACC17970.12362793289555608No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG17960.12355913604920349No Hit
GTCCCTCCGCCGAACATCCAATGATCACTAACACTATCCCACACCTGACA17840.12273357389297275No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17830.12266477704662018No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGC17790.12238958966120993No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT17730.12197680858309454No Hit
CTATTACTGTCAGGTGTGGGATAGTGTTAGTGATCATTGGATGTTCGGCG17660.12149523065862662No Hit
CTATCATCATAGACGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTA17660.12149523065862662No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17340.11929373157534458No Hit
CCCTGATTATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGT17190.11826177888005612No Hit
TAATCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCAT17170.118124185187351No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCC17150.11798659149464588No Hit
GGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGC16960.11667945141394717No Hit
GAATTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG16470.11330840594267158No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16460.11323960909631903No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16300.11213885955467802No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16100.11076292262762674No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATT16050.11041893839586395No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16040.11035014154951138No Hit
CCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAATT15890.10931818885422293No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC15560.10704789292458833No Hit
GGTCGGTCCCTCCGCCGAACATCCAATGATCACTAACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC15510.10670390869282553No Hit
GGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATTCCAATGTTGTTT15440.10622233076835758No Hit
GACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTA15420.10608473707565247No Hit
GTGATAATCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTC15220.10470880014860119No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA15030.1034016600679025No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG14980.10305767583613967No Hit
CTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGT14770.10161294206273584No Hit
GGATAGTGTTAGTGATCATTGGATGTTCGGCGGAGGGACCGACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14690.10106256729191533No Hit
GACTATTACTGTCAGGTGTGGGATAGTGTTAGTGATCATTGGATGTTCGG14660.10085617675285766No Hit
GTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG14580.10030580198203715No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14540.10003061459662689No Hit
TGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCA14540.10003061459662689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.046.1928441
GTATCAA27000.042.284511
GTGGTAT3200.041.910381
TGGTATC3250.040.199212
TATCAAC32200.035.2350922
ATCAACG32750.035.062073
TCAACGC33100.034.7951854
CAACGCA33500.034.3797235
AACGCAG34200.033.776576
GAAACAA50650.032.8073470
CGCAGAG37100.031.1309978
ACGCAGA37300.030.9640757
CAGAGTA37250.030.91335710
AGAGTAC37450.030.6564811
TAGCGCT450.004694835730.57026929
CTAGCGC450.004696416730.56816728
GCAGAGT38900.029.6021259
TACCTAT658.83657E-426.445946
AGAGCGT658.83657E-426.445946
AGTACGG36900.025.52418313
Produced by FastQC (version 0.11.5)