FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453555
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG116210.7994881514631369No Hit
GACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCACTA81380.5598687356171593No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCACTAACACTATCCCACAC80350.5527826604428453No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC73990.5090278661626151No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG57290.39413713275383455No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGG56470.3884957913529244No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51690.3556108987963992No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC47790.3287801287188995No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45820.31522714998744455No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTC43210.2972711730894256No Hit
GTGTTAGTGATCATTGGATGTTCGGCGGAGGGACCGACCTGACCGTCCTA43130.2967207983186051No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT43030.29603282985507945No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42340.29128584745675257No Hit
GTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC41630.2864012713657206No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40540.27890241511329117No Hit
CACTAACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGTGG40200.2765633223373041No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG40150.2762193381055412No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39010.268376497621349No Hit
GATTATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGG37580.2585385485929325No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGC36580.25165886395767617No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG36200.24904458379627878No Hit
ACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCACTAA35570.2447103824760673No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATT35250.24250888339278526No Hit
GTGTGGGATAGTGTTAGTGATCATTGGATGTTCGGCGGAGGGACCGACCTGACCGTCCTAGGTCAGCCCAAGG34710.23879385368974687No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACT34470.23714272937728534No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG33800.23253334067166362No Hit
GGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC32780.22551606234370217No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA32760.22537846865099703No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG32440.22317696956771502No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG32360.2226265947968945No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC32280.22207622002607402No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31760.21849878401574074No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG30910.21265105207577284No Hit
GGTGATAATCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT30680.21106872460966392No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30060.206803320135805No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA29360.20198754089112556No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28980.19937326072972816No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT28870.19861649541984996No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28870.19861649541984996No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCA28870.19861649541984996No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT28820.1982725111880871No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28160.19373191932881795No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA28020.1927687634798821No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26500.18231164283429246No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25950.1785278162849015No Hit
CGATTACCTGTGGGGGAAACAACATTGGAATTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC25740.17708308251149768No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC25220.17350564650116437No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25100.17268008434493362No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGAT24610.16930903887365803No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23860.1641492753972158No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23850.16408047855086325No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATTCCAATGTTGTTTCCCCCACAGGTAATCGTGGC23790.16366769747274784No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG23690.16297972900922222No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA23450.1613286046967607No Hit
CCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACA23120.15905830876712612No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGTGG22680.15603124752761335No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAATTAAAAGTGTG22470.1545865137542095No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAAACAA22460.15451771690785696No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22130.15224742097822236No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC22040.1516282493610493No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21760.14970193766317752No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21740.14956434397047239No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21280.14639968903825448No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21010.1445421741867353No Hit
CATCATAGACGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG20750.14275345618156865No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGG20490.14096473817640198No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20040.13786888009053663No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT19980.13745609901242128No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19960.13731850531971615No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19700.1355297873145495No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19410.13353467877032515No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19230.13229633553597903No Hit
CTTTTAATTCCAATGTTGTTTCCCCCACAGGTAATCGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG19020.1308516017625752No Hit
GTCCCTCCGCCGAACATCCAATGATCACTAACACTATCCCACACCTGACA18980.13057641437716497No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATCCAATGATCA18880.12988844591363932No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC18810.12940686798917136No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA18500.1272741657522419No Hit
GCTGGTACCAGTGCACACTTTTAATTCCAATGTTGTTTCCCCCACAGGTAATCGTGGCCGTCTTTCCTGGGGCCA18430.12679258782777397No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18270.12569183828613295No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18080.12438469820543427No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18040.124109510820024No Hit
GGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGC17890.12307755812473557No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACAT17870.12293996443203042No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGT17850.1228023707393253No Hit
ACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC17710.12183921489038942No Hit
CCCTGATTATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGT17470.12018809057792791No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17300.11901854418993434No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17210.11839937257276127No Hit
GGTCGGTCCCTCCGCCGAACATCCAATGATCACTAACACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC17090.1175738104165305No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGC17090.1175738104165305No Hit
CTATTACTGTCAGGTGTGGGATAGTGTTAGTGATCATTGGATGTTCGGCG17040.1172298261847677No Hit
CTATCATCATAGACGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTA16820.11571629556501131No Hit
AGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCC16750.11523471764054337No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16730.11509712394783825No Hit
CACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACC16670.11468434286972286No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16600.11420276494525491No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16400.11282682801820364No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16400.11282682801820364No Hit
GGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTG16350.11248284378644084No Hit
GAATTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG16350.11248284378644084No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16160.11117570370574213No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16030.1102813447031588No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC16030.1102813447031588No Hit
GGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATTCCAATGTTGTTT16000.11007495416410111No Hit
TAATCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCAT15850.10904300146881267No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15670.10780465823446654No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC15650.10766706454176142No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15600.1073230803099986No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATT15590.10725428346364603No Hit
CTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC15520.10677270553917809No Hit
CCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAATT15290.10519037807306914No Hit
GTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG15200.10457120645589607No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTCGGTCCCTCCGCCGAACATC15120.10402083168507556No Hit
CTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGT15020.10333286322154991No Hit
GGCTTCTGCTGGTACCAGTGCACACTTTTAATTCCAATGTTGTTTCCCCCACAGGTAATCGTGGCCGTCTTTC15010.10326406637519736No Hit
TGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCA14780.1016817389090884No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG14740.10140655152367815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG200.005269370556.3101368
TGCGTAT200.006159555654.1319967
GTACGAA354.9793057E-448.26650669
GTGGTAT4350.048.2546461
TGGTATC4500.045.8482782
GGTATCA14000.044.4886361
GTATCAA30400.037.2409631
GAAACAA50250.032.86699770
ATCAACG35700.031.272733
TCAACGC36000.031.0014574
CAACGCA36100.030.915585
TATCAAC36600.030.8160572
AACGCAG37200.030.1860336
CGCAGAG39250.028.5219488
AGAGTAC39200.028.38605311
TCGTGAG1604.8022102E-628.1151970
ACGCAGA40350.027.8295027
CAGAGTA40650.027.54058810
GCAGAGT41350.027.0734339
TTAACGG1404.783942E-1027.05687735
Produced by FastQC (version 0.11.5)