FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057938
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1050.046.1315339
TCGTATG1150.045.18457840
GGTATCA11100.044.899441
GTATCAA31600.042.3114971
CGACGTC1550.037.74215730
CTCGACG1600.036.54195828
TCTCGTA1350.035.85278338
TCAACGC38450.034.9457364
TGCCGTC1700.034.81735245
CGTATGC1600.034.67951241
ATCAACG38750.034.5865063
CAACGCA38950.034.5853655
GATACCG400.0025781134.54932437
ACTCGAC1700.034.384327
AACGCAG39750.033.8893136
TATCAAC39550.033.88692
TCGACGT1800.032.49096329
ACGCAGA42600.031.5399077
CGCAGAG43250.031.0658958
TAATCCG450.00453086830.79245440
Produced by FastQC (version 0.11.5)