FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057938
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19910.1881962837141685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.043.2915341
GTATCAA34050.040.1840551
TCAACGC40050.034.0169264
ATCAACG40300.033.905483
CAACGCA40450.033.7653775
TATCAAC40550.033.7203642
AACGCAG41500.033.078026
ACGCAGA44050.031.0852647
AGAGTAC44300.030.45222911
CGCAGAG45250.030.1850628
CAGAGTA45800.029.6741210
GCAGAGT46350.029.2465729
GTGGTAT5550.029.1133821
CGAGATA500.00677614628.35820863
AGTACGG31650.027.44085113
GAGTACG32450.026.8688612
CTTATCG1609.58702E-625.72007870
TACGGGG16950.025.31696515
TGGTATC6400.025.2300382
GTACGGG35150.024.51315314
Produced by FastQC (version 0.11.5)