FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670027
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT64800.38801767875609194No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58390.3496350657803736No Hit
CTCTACAGACTGCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA54880.32861744151441863No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT45650.2733488740002407No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40560.24287032485103532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35820.21448755020128416No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34230.2049667460466208No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG32980.19748183711999867No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC32440.1942483564636979No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31390.18796103296533528No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31330.1876017573368574No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31000.18562574138022916No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30420.18215274363827652No Hit
GTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTCTTTGGGTT28360.16981761372720322No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28130.16844039048470474No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA27550.16496739274275204No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA26300.15748248381612992No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC26200.15688369110200015No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG26090.1562250191164574No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAGAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA25940.15532683004526274No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25570.1531112970029826No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24400.14610542224766426No Hit
GGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTCTTTGGGTTCTAAT24080.14418928556244898No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT24050.14400964774821007No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24040.14394976847679708No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23070.1381414791497383No Hit
CTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC22690.13586606683604516No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22530.13490799849343754No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22170.13275234472257036No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21860.13089608730876806No Hit
CTACAGACTGCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG21690.12987813969474743No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC21590.1292793469806177No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA21400.1281416408237711No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21290.12748296883822835No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA21010.12580634923866502No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20770.12436924672475357No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG20760.12430936745334058No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20750.1242494881819276No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGGCCGTCACCC20570.12317166129649403No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTCTTTGGGTTCTAATCGGGCC20380.12203395513964745No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT20100.12035733554008408No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19820.11868071594052071No Hit
GCTCTACAGACTGCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19730.11814180249780393No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGCAGT19460.11652506216965354No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19250.11526759746998101No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC19040.11401013277030851No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA18970.11359097787041766No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18910.1132317022419398No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18710.11203411681368027No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18150.10868087761455353No Hit
TTATTACTGCATGCAAGCTCTACAGACTGCGTGGACGTTCGGCCAAGGGA17950.10748329218629399No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17750.10628570675803445No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17680.10586655185814361No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17630.10556715550107873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17460.10454920788705811No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17300.10359113954445048No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17000.10179476140206116No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16970.10161512358782224No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16840.10083669305945353No Hit
CAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTCTTTGGGTTCTAATCGGGC16780.10047741743097567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5900.042.6022342
GGTATCA14950.041.229911
GTGGTAT6250.040.7710841
GTATCAA35450.037.8833961
TATCAAC40800.032.575322
ATCAACG42800.031.0531083
TCAACGC43400.030.6238044
TAAGGCG802.7865517E-630.15413329
CAACGCA44250.030.0355475
AACGCAG44600.029.7998456
CGCAGAG49350.026.9307638
AGAGTAC49800.026.4108611
CAGAGTA50350.026.39589310
CTGTCTT32250.025.30502970
TGTTAGA11200.025.22247770
ACGCAGA53100.024.9640337
CGAACTA2100.024.61561829
TATTCTA2952.562956E-923.40797870
AGTACGG46300.022.8313
GAGTACG46400.022.78079812
Produced by FastQC (version 0.11.5)