FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421794
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45220.31804888753223043No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG37810.2659316328525792No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32650.22963945550480588No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29930.2105086953524913No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29600.20818768401048254No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29070.20445999912786242No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28850.20291265823318988No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28700.20185765307773137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28250.19869263761135578No Hit
GTATGATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT28220.19848163658026408No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27540.19369894654218545No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCAACTATTTAACTTGGTATCAGAAGAAA27500.19341761183406317No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27450.19306594344891032No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27030.19011192901362645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26430.18589190839179234No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26080.18343022969572245No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25600.18005421319825515No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24800.1744275190358097No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG24610.1730911791722289No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23480.16514347366777465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23200.16317413071091874No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT23050.1621191255554602No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22550.15860244170393178No Hit
GTCTCCATCCTCCCTGCCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCAA21870.15381975166585313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21280.1496700647210496No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20780.14615338086952118No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG20200.1420740276017482No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19790.1391903468434949No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAGTAGATTTTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG19660.13827600904209753No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC19620.13799467433397525No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT19410.1365176671163333No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19380.1363066660852416No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19190.1349703262216608No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19110.13440765680541625No Hit
CAATAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATA18930.13314165061886601No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC18870.1327196485566826No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18790.13215697914043806No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA18630.13103164030794898No Hit
GCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAAGT18460.12983596779842932No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAAAATCTAC18050.126952287040176No Hit
GCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGG17990.1265302849779926No Hit
GTACTATGTGGACTCTGTGAAGGGACGAGTCACCATCTCCAGAGACAACG17800.12519394511441181No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC17770.1249829440833201No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT17480.1229432674494336No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17420.1225212653872502No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17350.12202892964803622No Hit
ATATTACTGTCAACAGTATGATAATCTCCCGCTCACTTTCGGCGGAGGGA17340.12195859597100564No Hit
ATAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA17220.12111459184663884No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17090.12020025404524143No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17030.11977825198305801No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16900.11886391418166065No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16880.1187232468275995No Hit
AGTCAGGACATTAGCAACTATTTAACTTGGTATCAGAAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACG16870.11865291315056893No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16580.11661323651668244No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16540.11633190180856016No Hit
CAGTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATCCCAGATCCACTTCCA16070.11302621898812346No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT15770.1109162086772064No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15630.10993153719877843No Hit
CCACTGAACCGTGATGGGACCCCTGCCTCCAAATCGGATGCAGCGTAGAT15620.10986120352174789No Hit
GTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATCCCAGATCCACTTCCA15570.10950953513659503No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT15250.10725885747161686No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT14840.10437517671336353No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC14810.10416417568227182No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14810.10416417568227182No Hit
GTGAAGGGACGAGTCACCATCTCCAGAGACAACGGCGAGAACTCACTGTA14780.10395317465118013No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14770.10388284097414956No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14680.10324983788087445No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14590.10261683478759932No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14400.10128049492401853No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC14320.10071782550777399No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14300.10057715815371285No Hit
GTTCAGTGGAAGTGGATCTGGGATACATTTTACTTTCACCATCAGCAGCC14270.10036615712262113No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14250.10022548976856001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTT200.003737691361.41246469
CGCTTAT200.007317293451.82513824
GTATCAA20550.040.8649181
TATCAAC23650.035.645762
TCAACGC25200.033.863384
CAACGCA25250.033.7975165
ATCAACG25300.033.7295343
AACGCAG25800.033.3448526
GTGGTAT2500.033.1762541
GTAGTTT2800.031.1489970
AGAGTAC27550.031.09479111
CAGAGTA28550.030.00565710
CGCAGAG28650.029.9009258
GCAGAGT29950.028.718399
ACGCAGA30750.027.858917
ACTACTC500.00768886527.6342418
CGAACTA1653.6379788E-1227.22607429
GAGTACG27550.024.44867312
AGTACGG27500.024.24191313
TTCGGGG14900.023.83211370
Produced by FastQC (version 0.11.5)