FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421794
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42200.29680811706899873No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG38920.2737386710029723No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34440.24222918369327767No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29500.20748434724017686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27930.19644195994637761No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27120.19074493210690158No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCAACTATTTAACTTGGTATCAGAAGAAA26630.18729858193240373No Hit
GTCTCCATCCTCCCTGCCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCAA26190.1842039001430587No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26140.18385223175790585No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26140.18385223175790585No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25820.18160155409292766No Hit
GTATGATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT25580.17991354584419403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25480.17921020907388835No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25420.17878820701170492No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24340.17119216989240355No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24300.17091083518428127No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23560.1657061430840192No Hit
GATCTACGCTGCATCCGATTTGGAGGCAGGGGTCCCATCACGGTTCAGTGGAAGTGGATCTGGGATACATTTTA23430.16479180528262183No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG23070.16225979290952133No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22860.16078278569187943No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22720.15979811421345144No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG21630.1521317434171195No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT20760.14601271351546005No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20590.1448170410059404No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20510.14425437158969584No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20450.1438323695275124No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAGTAGATTTTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG19490.13708033653257787No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19270.13553299563790536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19090.13426698945135512No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG19070.134126322097294No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18630.13103164030794898No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18550.13046897089170442No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC18240.1282886269037568No Hit
CAATAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATA17940.12617861659283974No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17850.12554561349956464No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17840.12547527982253406No Hit
GTACTATGTGGACTCTGTGAAGGGACGAGTCACCATCTCCAGAGACAACG17760.12491261040628951No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17700.12449060834410612No Hit
AGTCAGGACATTAGCAACTATTTAACTTGGTATCAGAAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACG17550.12343560318864759No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT17540.12336526951161701No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17420.1225212653872502No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17280.12153659390882222No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT17230.12118492552366938No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT17130.1204815887533637No Hit
ATATTACTGTCAACAGTATGATAATCTCCCGCTCACTTTCGGCGGAGGGA17050.11991891933711916No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17050.11991891933711916No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16940.11914524888978291No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAAAATCTAC16930.11907491521275233No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16700.11745724064104927No Hit
CAGTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATCCCAGATCCACTTCCA16310.11471422723685709No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC16160.11365922208139856No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT16030.11274488428000118No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15860.11154921177048151No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15710.11049420661502299No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15700.11042387293799243No Hit
ATAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA15630.10993153719877843No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15630.10993153719877843No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15570.10950953513659503No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15490.10894686572035048No Hit
GCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGG15370.10810286159598367No Hit
GCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAAGT15340.10789186056489196No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC15170.1066961880553723No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15120.10634451967021946No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC15090.10613351863912775No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15070.10599285128506661No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15020.10564118289991377No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA14990.10543018186882207No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14980.1053598481917915No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14880.10465651142148583No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT14850.10444551039039411No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14780.10395317465118013No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14590.10261683478759932No Hit
CCACTGAACCGTGATGGGACCCCTGCCTCCAAATCGGATGCAGCGTAGAT14540.10226516640244648No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14420.10142116227807967No Hit
GTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATCCCAGATCCACTTCCA14340.10085849286183511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)