FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132787
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40240.35523006531678064No Hit
GAGTTCACCCTCACCATCAATATGCTGGAAACTGAAGATGCTGCAACGTATTACTGTCATCAGAGTCGGAGTTTA35410.3125918641368589No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT32650.28822717774833223No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27000.2383501929312395No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25760.22740373962624924No Hit
GTATTACTGTCATCAGAGTCGGAGTTTACCAGGGACTTTTGGCCAGGGGA24530.21654556417049275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23390.20648188935784043No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22800.20127349625304666No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22660.2000376063637736No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22590.19941966141913706No Hit
ATTATAACCCGTCCCTCAAGAGTCGAGTTTCCATATCACGAGACACGTCC22160.19562371390208394No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21750.1920043220834985No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21330.1882966524156792No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC21010.18547176124019785No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20560.1814992580246772No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19910.17576119782448069No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19560.17267147310129793No Hit
GTGTAAGCTATTACCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTT19130.16887552558424487No Hit
CAGTAATACGTTGCAGCATCTTCAGTTTCCAGCATATTGATGGTGAGGGTGAACTCTGTCCCAGATCCACTGCCA18820.16613891225799732No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18760.16560924516259454No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18680.16490302236872423No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG17680.15607523744534496No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17550.1549276254053057No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17440.15395656906373395No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16940.14954267660204434No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16360.14442256134648437No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15850.13992039103556095No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15530.1370954998600796No Hit
GGTTATAATAGGTGCTCCCACTGTAATAGATGAACCCAATCCACTCCAGG14960.13206366245375342No Hit
ATCTGGGACAGAGTTCACCCTCACCATCAATATGCTGGAAACTGAAGATG14000.12358898892730938No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13920.12288276613343903No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13910.12279448828420524No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13860.12235309903803628No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13770.12155859839493215No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13760.12147032054569835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13640.12041098635489283No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13550.1196164857117887No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13520.11935165216408734No Hit
GTCTCTGGTGGCTCCATCAGCACTGGTGATTACTACTGGAGTTGGATCCG13510.11926337431485354No Hit
GGGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT13410.11838059582251562No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT13260.11705642808400873No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13000.11476120400393013No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12980.11458464830546254No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACCCTCACCATCAATATGCTGGAAA12950.11431981475776117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCCTGGTAAACT12870.11361359196389084No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12850.11343703626542324No Hit
GGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCT12800.11299564701925428No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA12550.11078870078840948No Hit
GTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCTGAGAAGGACTG12150.10725758681905777No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12090.10672791972365502No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11960.10558030768361572No Hit
GTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACCCTCACCATCAATATG11850.104609251342044No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11480.10134297092039368No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11480.10134297092039368No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11370.10037191457882197No Hit
ACGTATTACTGTCATCAGAGTCGGAGTTTACCAGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11370.10037191457882197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)