FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132787
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38790.3424297771778807No Hit
GAGTTCACCCTCACCATCAATATGCTGGAAACTGAAGATGCTGCAACGTATTACTGTCATCAGAGTCGGAGTTTA33410.29493629429010043No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT31500.27807522508644605No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27050.23879158217740845No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23660.20886539128715287No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23170.20453977667469703No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22230.19624165884672054No Hit
GTATTACTGTCATCAGAGTCGGAGTTTACCAGGGACTTTTGGCCAGGGGA22050.19465265756051225No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21980.1940347126158757No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21520.18997393155112124No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21380.18873804166184815No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC21300.18803181886797785No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20610.18194064727084616No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20510.18105786877850824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19390.17117074966432347No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17830.15739940518385187No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17810.1572228494853843No Hit
GTGTAAGCTATTACCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTT17650.1558104038976436No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17520.1546627918576043No Hit
GGGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT17360.15325034626986364No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17180.15166134498365536No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17050.15051373294361606No Hit
CAGTAATACGTTGCAGCATCTTCAGTTTCCAGCATATTGATGGTGAGGGTGAACTCTGTCCCAGATCCACTGCCA16500.14565845123575746No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15910.14045005813096373No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15350.13550649857387134No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15270.134800275780001No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15070.13303471879532516No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15010.13250505169992238No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14420.12729665859512865No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG14350.1266787136504921No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14120.12464832311811487No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14050.12403037817347834No Hit
GGTTATAATAGGTGCTCCCACTGTAATAGATGAACCCAATCCACTCCAGG13740.12129376484723076No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13500.11917509646561975No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13320.11758609517941149No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12990.11467292615469633No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA12740.11246597992385153No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12410.10955281089913638No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12390.10937625520066878No Hit
GTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACCCTCACCATCAATATG12320.10875831025603225No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12300.10858175455756468No Hit
GTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCTGAGAAGGACTG12240.1080520874621619No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCCTGGTAAACT12190.10761069821599294No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACCCTCACCATCAATATGCTGGAAA12190.10761069821599294No Hit
GTCTCTGGTGGCTCCATCAGCACTGGTGATTACTACTGGAGTTGGATCCG12170.10743414251752537No Hit
ATCTGGGACAGAGTTCACCCTCACCATCAATATGCTGGAAACTGAAGATG12110.1069044754221226No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12110.1069044754221226No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11870.10478580704051157No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11860.1046975291912778No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT11830.10443269564357643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11750.10372647284970608No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11640.10275541650813436No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11490.10143124876962747No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11370.10037191457882197No Hit
GGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCT11370.10037191457882197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3400.048.705322
GTGGTAT3550.046.696871
GGTATCA9100.039.469971
GTATCAA20800.038.521171
ACGCGGT400.002503852434.75604250
TATCAAC23250.034.2770232
ATCAACG24250.032.8446733
TCAACGC24650.032.303144
CAACGCA24900.031.9788115
AATCGAG800.00396457331.64210970
AACGCAG25500.031.091196
CGCAGAG27550.028.9015338
AGAGTAC27750.028.698311
CAGAGTA27850.028.59020610
ACGGAAC1253.1777745E-928.29986666
TAGCGCC500.00776871727.57531410
GCAGAGT29200.027.5044889
ACGCAGA29150.027.1981247
CAACGGA1305.2350515E-927.00587364
TAGAGTG951.0577056E-525.3994395
Produced by FastQC (version 0.11.5)