FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353623
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44500.32874736909759955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34780.2569400785890902No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT33750.24933086982121314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33500.24748397448920417No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31500.23270881183313227No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28690.21194970830135124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28340.2093640548365387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27930.20633514649204396No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27610.20397112046707244No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24130.17826233744550737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24100.17804071000566626No Hit
GAGTTACAGAACTCCTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23920.17671094536661983No Hit
CCATCAGCACTCTGCAACCTGAAGATATTGGAACTTACTACTGTCAACAG23590.17427304352836795No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22590.16688546220033199No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22040.16282229246991223No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21860.16149252783086576No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA20730.15314456093018514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20610.15225805117082084No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20480.15129766559817617No Hit
GTTTAGGGGCCGTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA20250.14959852189272788No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20210.14930301863960643No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20180.14908139119976538No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19020.14051179685924367No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18940.13992079035300078No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAACTATTTAAATTGGTATCAGCAGAAA18800.13888652896707576No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18640.13770451595459002No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18410.13600537224914175No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17890.13216382995856307No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17880.13208995414528268No Hit
CCATAGATCAGGAGTTTAGGGGCCGTCCCTGGTTTCTGCTGATACCAATT17870.13201607833200232No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17390.1284700392945451No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16930.12507175188364855No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16500.12189509191259308No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16230.11990044495402338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16120.11908781100793944No Hit
GATTTCACTCTCACCATCAGCACTCTGCAACCTGAAGATATTGGAACTTACTACTGTCAACAGAGTTACAGAACT15460.11421200733143572No Hit
GCCCCTAAACTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT14750.10896682458853019No Hit
GTTACAGAACTCCTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14710.10867132133540874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14700.10859744552212838No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14670.10837581808228731No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT14580.10771093576276408No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCTTCC14440.10667667437683905No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14220.10505140648467114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14180.10475590323154971No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAA14090.10409102091202646No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13950.10305675952610144No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13650.10084048512769064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6100.036.147081
GTATCAA29900.036.0658571
CGATTAT501.8035901E-434.6863145
TCGATTA553.1608812E-431.52478444
TATCAAC34700.030.9501442
TCAACGC35650.030.2904974
ATCAACG35800.029.885333
CAACGCA36800.029.4362835
AACGCAG36900.029.2633156
TATCGGC500.00782117327.5376912
TGTTAGA12000.027.52597670
AGAGTAC39650.027.14797411
CGCAGAG40200.026.8611038
ACGCAGA42050.025.7611247
ACGGGGG6800.023.7768116
AGTACGG37900.023.32267213
GGTACCG1502.9884177E-822.9260376
GAGTACG38450.022.80762912
TACGGGT3550.022.28434615
GTTCGCC800.002953759421.49395411
Produced by FastQC (version 0.11.5)