FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780550
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19470.2494394977900199No Hit
GTATTACTGTCAACAGTATGGTGACTCACCTACGACGTTCGGCCAAGGGA13540.17346742681442573No Hit
GTGTATTACTGTCAACAGTATGGTGACTCACCTACGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12060.15450643776824036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11710.1500224200883992No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11530.14771635385305235No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11510.14746012427134714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10770.13797962974825445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10310.13208634936903466No Hit
GTCTGGGACAGACTTCACTCTCACCATCGGCAGACTGGAGCCTGAAGATC10060.12888347959771956No Hit
GTAATACACTGCAAGATCTTCAGGCTCCAGTCTGCCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9560.12247774005508937No Hit
GTATGGTGACTCACCTACGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT9380.12017167381974249No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9070.11620011530331176No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9060.11607200051245915No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGAAACTACTTAGCCTGGTACC8670.1110755236692076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8550.10953814617897636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8320.10659150598936647No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8080.10351675100890398No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC8000.10249183268208316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7820.10018576644673627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.042.6332781
GTATCAA22550.037.59481
TATATCG557.473838E-637.575535
CAACGCA26300.031.8249035
TCAACGC26300.031.8249034
TCGTATG553.1883345E-431.47626340
TATCAAC26600.031.4679932
ATCAACG26700.031.3481273
ATATCGC553.2871094E-431.3129436
AACGCAG26900.031.1150576
GCCTAAT1453.9146526E-628.85866270
CGGGATT1202.7266651E-928.70536817
ACGCAGA29450.028.5378427
CGCAGAG29550.028.4412638
AGAGTAC29550.028.32651711
TACGTAG500.007440611727.8191347
GCAGAGT31400.026.7655879
CTCGTAT658.491235E-426.62518739
CAGAGTA31650.026.4453410
GTATACC1651.1641532E-1025.0631981
Produced by FastQC (version 0.11.5)