FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853297
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68680.37058280459095333No Hit
GAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCAT49500.26709156708287984No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45560.24583215750092943No Hit
GTCCTGATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTT41520.22403316899557926No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41360.2231698427181396No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC39130.211137232726325No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36680.197917549103031No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35820.19327717036179307No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35210.18998573892905454No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34950.1885828337282152No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTG34860.1880972126971554No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAACTATTTAAATTGGTATCAGCAGAAA33940.1831330866018776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33560.18108268669295854No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32760.17676605530576048No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31710.17110047661006303No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAA30170.1627909611897068No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT29880.1612261823118475No Hit
GATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA28860.15572247729316996No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTC28530.15394186684595076No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27600.14892378285833302No Hit
GGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCA26640.14374382519369533No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26580.1434200778396555No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26130.14099197268435656No Hit
GTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCA25810.13926532012947737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25450.13732283600523823No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTGAACGGGTTA25350.13678325708183847No Hit
GGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT25330.13667534129715853No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25220.1360818044814188No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25070.13527243609631914No Hit
GTTTAGTCCCCTGGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAG24120.13014643632402145No Hit
ACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGA23980.1293910258312618No Hit
ATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA23280.1256139733674635No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23180.12507439444406374No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22570.12178296301132523No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20930.11293386866756919No Hit
ACTTACTACTGTCAACAGAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAA20910.11282595288288925No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAAC20870.11261012131352935No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA20800.11223241606714952No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20490.11055972140461028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19930.10753807943357163No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCC19840.10705245840251185No Hit
GGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19550.10548767952465254No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA19540.10543372163231257No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19360.10446247957019301No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19340.10435456378551305No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19270.10397685853913323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19150.10332936383105353No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC18960.10230416387659398No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18950.102250205984254No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18930.10214229019957406No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18640.10057751132171476No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA18610.10041563764469483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA350.007146178752.1352670
GTATCAA53300.040.9505351
GTGGTAT6550.037.3176771
TATCAAC60100.036.0802762
TCAACGC62600.034.9133454
ATCAACG62550.034.7761763
CAACGCA63400.034.418515
AACGCAG65150.033.493996
TCGACGT1750.031.49639929
ACGCAGA70650.030.9310787
AGAGTAC71000.030.92401111
CGCAGAG70950.030.800298
GCAGAGT74350.029.4843779
CAGAGTA76850.028.57000410
ACCGATA500.0069054128.24982364
TGGATCG9300.028.12314470
AGTACGG54150.027.77259413
GAGTACG54500.027.65738312
TATATCG500.007825904527.534815
CGACGTC1900.027.20627830
Produced by FastQC (version 0.11.5)