FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853297
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65020.3508342159945222No Hit
GAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCAT47860.2582424727391238No Hit
GTCCTGATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTT43970.23725285261887327No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG39640.2138890852356638No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39200.21151493797270488No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC38490.2076839276165666No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38320.20676664344678697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37770.2037989593680883No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTG35880.19360091771583293No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAACTATTTAAATTGGTATCAGCAGAAA35590.1920361388379736No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35370.19084906520649417No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34140.18421224444867715No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32870.17735959212150024No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31320.168996118808804No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAA31020.16737738203860472No Hit
GATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA30200.1629528348667267No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT28720.15496706680041028No Hit
GGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCA27570.14876190918131307No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27360.14762879344217358No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTC27330.14746691976515366No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27280.1471971303034538No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26660.1438517409783753No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26200.1413696779307364No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25370.13689117286651842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25110.13548826766567906No Hit
GTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCA24970.1347328571729194No Hit
GGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT24290.13106372049380105No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTGAACGGGTTA24040.12971477318530167No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23740.1280960364151024No Hit
ATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA23230.12534418390576363No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22770.12286212085812474No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22360.12064984727218574No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22350.12059588937984576No Hit
GTTTAGTCCCCTGGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAG22250.12005631045644599No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA22030.11886923682496653No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21840.117844036870507No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21420.11557780539222802No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAAC21230.11455260543776846No Hit
ACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGA20860.11255616342118938No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20830.11239428974416944No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20760.11201658449778963No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCC20750.11196262660544964No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA20460.11039784772759036No Hit
GGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20010.10796974257229143No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19680.10618913212507224No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19450.1049481006012528No Hit
ACTTACTACTGTCAACAGAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAA18950.102250205984254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52600.042.70711
TATCAAC60550.036.8486442
GTGGTAT6000.036.6945341
ATCAACG61800.035.963893
TCAACGC61950.035.8622864
CAACGCA63000.035.261735
AACGCAG63600.034.983066
AGAGTAC69600.032.1178711
CGCAGAG69750.032.045348
ACGCAGA69850.031.902027
GCAGAGT72700.030.8866989
CAGAGTA74750.030.0420710
AGTACGG53500.028.11754413
GAGTACG54200.027.75215512
CGATTGT1606.3279076E-627.13651570
ATGCGTA1501.178887E-825.02595168
AGTACTT30650.023.97951313
GTACGGG62950.023.8972114
GAGTACT30900.023.78357912
TACGGGA25500.023.70827115
Produced by FastQC (version 0.11.5)