FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494909
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42600.038.3251341
GGTATCA18300.035.6863561
TATCAAC49800.032.502642
ATCAACG51500.031.5621413
TCAACGC52350.030.9840264
CAACGCA53000.030.7995785
GTGGTAT8100.030.552531
TGGTATC8150.030.3600122
AACGCAG53850.030.2496036
ACGCAGA57550.028.3029067
TCGTATG1253.985406E-927.70677240
AGAGTAC58150.027.5972111
CGCAGAG59000.027.549088
CAGAGTA58950.027.51415410
GCAGAGT63200.025.7182879
AGTACGG47200.025.4077913
GAGTACG47650.025.23995812
TACGGGG22950.022.45908415
GTACGGG54000.021.89012114
ACGAACC3550.021.65645452
Produced by FastQC (version 0.11.5)