FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494909
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22880.15305279451792717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49650.039.6726951
TATCAAC56150.034.684912
ATCAACG56950.034.0633663
GGTATCA20900.033.7181631
TCAACGC57800.033.546724
CAACGCA58000.033.4902085
AACGCAG58550.033.175616
ACGCAGA62050.031.193697
CGCAGAG62400.030.9637248
AGAGTAC62750.030.574311
CAGAGTA62900.030.5539110
GCAGAGT67100.028.9994649
AGTACGG53100.027.15554613
TGGTATC8500.027.0746672
GTGGTAT8900.027.0373671
GAGTACG53850.026.77106312
TACGGGA19150.023.12806115
TACGCGA750.002050554822.88824514
GTACGGG62450.022.81494314
GAGTACT22100.021.74391712
Produced by FastQC (version 0.11.5)