FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833001
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.041.2003671
GTATCAA17050.036.2637441
TTTAACG2000.034.57077834
TTAACGG2150.032.17046435
TTCGGTA553.235544E-431.3977972
TATTAGA2650.031.2793162
TCAACGC20250.030.3589554
TAACGGC2300.030.08143236
ATCAACG20800.029.722243
CAACGCA21250.028.93035
CATAAGC854.3888867E-628.442712
TATCAAC22250.028.0957372
AACGCAG22200.027.6922916
CTAACCG2550.027.29969252
ACGCAGA23050.026.819337
TAACCGT2600.026.77955853
GTATTAG3300.026.1679731
CGCAGAG23800.025.9741828
AGAGTAC23200.025.90162811
CAGAGTA23200.025.90162810
Produced by FastQC (version 0.11.5)