FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833001
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18100.043.716541
GGTATCA7250.040.5107041
TCAACGC20950.037.539034
CGGTCTA400.001875421436.86300368
ATCAACG21550.036.6693273
CAACGCA21500.036.5787285
AACGCAG22150.035.505316
TATCAAC23150.034.1636662
CGCAGAG23750.033.1133738
ACGCAGA23850.032.9745337
AGAGTAC24150.032.56686411
CAGAGTA24550.032.31532310
GCAGAGT26500.030.0675669
AGTACGG22200.027.35080513
GAGTACG22650.026.80419212
GTATAGG1001.5444322E-524.1872751
TACGGGG10700.023.86076715
GTGGTAT2800.023.4468461
GGTACGA750.00198030523.0244222
GTTAGCC902.1225036E-423.0050623
Produced by FastQC (version 0.11.5)