FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002077
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27550.039.926911
GGTATCA11000.036.049711
GTGGTAT4600.035.2319451
ATCAACG32700.033.5282553
TCAACGC33450.032.879574
TATCAAC33750.032.6894652
CAACGCA33950.032.3953365
AACGCAG34500.031.8788856
CTCGGTA450.00463174730.65401827
ACGCAGA37100.029.4545157
CGCAGAG37550.029.1933358
AGAGTAC37650.028.5650211
CAGAGTA38450.028.23963710
GCAGAGT40300.027.1143449
CGACGTT700.001089820225.51143367
TGGTATC6250.025.3751962
ACGGGTA2650.024.71454616
AGTACGG32900.023.7834513
GAGTACG33150.023.60408812
AGTACTT13650.022.22260313
Produced by FastQC (version 0.11.5)