FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002077
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11450.11426267642107343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28450.040.679261
GGTATCA12200.036.42051
TATCAAC33500.034.3207932
TCAACGC33950.034.1393664
ATCAACG33950.033.8422163
CAACGCA34300.033.791015
GTGGTAT5100.033.768821
AACGCAG35750.032.5166666
CGCAGAG39200.029.741128
ACGCAGA39150.029.6927417
AGAGTAC39400.029.24392111
CAGAGTA40050.028.76786810
CCGCATA500.00716303328.03740958
GCAGAGT42600.027.5288759
GAATCCG1200.001054308525.65440870
AGTACGG34700.025.38083813
GAGTACG35250.025.08116712
TGGTATC6800.024.8052042
ACGGGCT2700.024.21188716
TACGGGC6450.023.47100815
Produced by FastQC (version 0.11.5)