FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178959
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24350.040.8406451
CAACGCA28300.034.602245
ATCAACG28650.034.431913
GGTATCA10100.034.1883161
TCAACGC28900.033.8838584
TATCAAC29300.033.6937942
GTGGTAT5100.033.1760751
AACGCAG29750.032.915756
ACGCAGA32100.030.3986137
AGAGTAC32000.029.85089911
CGCAGAG33150.029.4357598
TGGTATC5400.029.4046232
CAGAGTA33850.028.42200910
TACCGTA658.685992E-426.523397
GCAGAGT37500.026.2051099
GAGTACG28150.025.24004612
AGTACGG28350.025.06304713
GTATAGG2500.024.8617421
TACCGTC700.001338289524.628867
AATAGCG3150.024.45915662
Produced by FastQC (version 0.11.5)