FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548183
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20530.13260706260177252No Hit
GTAATACACGGCCGTGTCTGCGGCACTCACAGAACTCAACTTCAGGGAGA17080.11032287526732952No Hit
GTCTCTGGTGGCTCCATCAGCAATAATGGTGATTACTATTGGAGTTGGAT16860.10890185462571285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15660.10115083294416746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3500.039.4525381
GTATCAA26950.039.3244441
TATCAAC31700.033.2066732
TCAACGC32900.032.308114
ATCAACG33100.031.9054013
CAACGCA33600.031.6370645
AACGCAG34200.031.082036
CGATTAG450.004613832630.67935825
CGATTGT1054.4648544E-429.71902870
AGAGTAC37700.028.19369911
GGTATCA19450.027.5102831
CGCAGAG39350.027.0114978
ACGCAGA40250.026.3217757
GCAGAGT40800.026.2206979
TACTGAA8800.023.40373670
AGTACGG38000.023.06634313
CGTAAGA750.001988916423.0087762
GAGTACG38450.022.88539912
TATTAGA1854.820322E-1022.3869172
CAGAGTA49700.021.3169310
Produced by FastQC (version 0.11.5)