FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548183
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19130.12356420397330291No Hit
GTCTCTGGTGGCTCCATCAGCAATAATGGTGATTACTATTGGAGTTGGAT17270.11155012036690753No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16340.10554307856370986No Hit
GTAATACACGGCCGTGTCTGCGGCACTCACAGAACTCAACTTCAGGGAGA16140.1042512416167856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27050.039.0565071
GTGGTAT5350.036.7841151
TATCAAC32200.032.897952
TCAACGC33750.031.1362694
ATCAACG34050.030.9761333
CAACGCA34750.030.2412365
AACGCAG35350.029.9228866
TGTTAGA5050.028.59278570
TACTGAA8100.027.90213870
AGAGTAC39650.026.76640911
CGCAGAG40650.026.1910238
AATCGAG1459.304597E-525.97785270
ACGCAGA41300.025.7788147
GCAGAGT43600.025.0511289
CGAACTA1951.8189894E-1224.75304424
TATACGA700.001320089224.68739936
GGTATCA19750.022.5507951
GCGTAGA11700.021.46318270
GCGAACT2251.0913936E-1121.44986523
GAGTACG38550.021.36338612
Produced by FastQC (version 0.11.5)