FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744353
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.036.069051
GTATCAA22200.035.9972461
CGTATGC1001.3842509E-831.19497941
CTCGTAT1001.4035322E-831.1505639
AACGAAT450.00450250730.8307942
TCGTATG1052.2431777E-829.68531840
CAACGCA27250.029.5739465
TATCAAC27050.029.5390552
GTGGTAT3950.029.522161
ATCAACG27200.029.5022343
TCAACGC27700.029.0935064
AACGCAG27800.028.8654966
TGGTATC4150.028.0956292
ACGCAGA30000.026.7468957
CAGAGTA29600.026.41325210
CGCAGAG30600.026.3345078
AGAGTAC29700.026.32431811
TCTCGTA1208.306961E-825.93945538
GCAGAGT32100.024.8902749
AGGGTCG700.001380942124.495145
Produced by FastQC (version 0.11.5)