FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023061
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18350.040.499371
GGTATCA8300.037.4809271
TGCGATA400.00254016134.65385439
ATCAACG21500.033.9143643
CAACGCA21950.033.3797875
TATCAAC22300.033.007642
TCAACGC22250.032.9297184
ATACCGA553.223396E-431.4187936
AACGCAG23750.030.9954666
ACGCAGA25300.028.9528547
AGAGTAC26200.027.69182611
CGCAGAG26800.027.4612858
CGAACTA1800.026.88972529
CAGAGTA27500.026.38533810
GCAGAGT30400.024.2092919
AATAGCG1953.092282E-1123.29556862
TGAGTCG1300.001919363723.14712770
CAACCGA2550.023.1445544
AGTACGG22750.023.08318713
ACCGGTC750.001975421823.0348198
Produced by FastQC (version 0.11.5)