FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023061
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18550.040.2365881
GGTATCA8000.035.8508451
CAACGCA22450.033.036185
TCAACGC22900.032.3869974
ATCAACG23000.032.2556463
TATCAAC23150.031.919442
AACGCAG24200.030.7897456
GTGGTAT3350.029.9133111
ACGCAGA25200.029.4310427
CGCAGAG26850.027.4939548
AGAGTAC26400.027.44530111
CAGAGTA27650.026.19943210
CCGGTTA700.001325189824.67008826
GCAGAGT30250.024.2896889
TGGTATC3850.023.3187432
AGTACGG24150.023.2897913
GAGTACG24400.023.19258312
GCCGTTA1554.0778104E-822.26196115
GTATAAG3150.021.9397051
GTGTTAG2451.8189894E-1221.1561431
Produced by FastQC (version 0.11.5)