FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565907
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18060.3191337092490462No Hit
GTAATACACGGCCGTGTCCCCGGCTCTCAGGCTGTCCATTTGCAGATACA14660.25905316597956907No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13560.23961534315709115No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT12630.22318154749808713No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12270.2168200782107307No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11710.20692445931928746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11570.20445055459642664No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11140.19685213294763979No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11090.19596859554661805No Hit
GTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11080.19579188806641373No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCTACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG11060.19543847310600507No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT9880.1745869904418924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9490.16769539871392297No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9470.16734198375351428No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9310.16451466407024476No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9290.16416124910983607No Hit
GTGTATTACTGTGCGAGAGAGAGGAGGGGGTTCTTGTATGCTTTTGATAT9230.16310100422860999No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9190.16239417430779263No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8770.15497246013921018No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG8260.14596037864878858No Hit
GAGCTACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG8120.14348647392592775No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT7870.13906878692081914No Hit
GTCTTTGTCTCCAGGGGAAAGAGCTACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA7810.1380085420395931No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7770.1373017121187757No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7640.13500451487611922No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7620.13465109991571053No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7480.1321771951928497No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7340.12970329046998888No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7300.12899646054917152No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCGCTCACTTTCGGCGGAGGGA7280.1286430455887628No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT7200.12722938574712808No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7160.12652255582631067No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7130.12599243338569766No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7000.12369523614304118No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6920.12228157630140642No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6880.12157474638058903No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6850.121044623939976No Hit
CAGTAATACACGGCCGTGTCCCCGGCTCTCAGGCTGTCCATTTGCAGATA6760.1194542566181369No Hit
GCGTAGCAACTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAACCGAACTGTGGCTGCACCAT6740.11910084165772822No Hit
CTCCTGTGCAGCCTCTGGAATCAGCTTTAGTAACTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG6720.11874742669731952No Hit
GGGTATTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGACGTCCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGC6570.11609681449425435No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCC6550.11574339953384566No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6490.11468315465261959No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6330.11185583496935009No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6330.11185583496935009No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGGA6320.11167912748914574No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6320.11167912748914574No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6260.11061888260791967No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA6240.11026546764751098No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6190.10938193024648925No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG6180.10920522276628492No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6130.1083216853652632No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA6090.10761485544444581No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG6070.10726144048403713No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5960.10531765820178934No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA5960.10531765820178934No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC5900.10425741332056328No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT5890.10408070584035892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5850.10337387591954154No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT5820.10284375347892852No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTAC5790.10231363103831548No Hit
GTCCACATAGTATGTCTCACTTCCATCTTGCTTTATATTGGACACCCACT5730.10125338615708943No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5660.100016433795659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC200.007305276651.84261335
GTCTAGA352.2557657E-549.343321
GTATCAA9800.043.7040821
TATCAAC11700.036.2859272
ATCAACG11800.035.6638263
TCAACGC12200.034.1936384
CAACGCA12350.033.7753455
AACGCAG12650.032.9772646
GGTATCA3800.032.722411
GTATTAG553.2327266E-431.4002971
ATGTTAG450.004387251630.99334760
CGCAGAG14250.029.516498
TGGTATC2900.028.5648672
AGAGTAC14550.028.19705611
GTGGTAT2950.028.1006031
ACGCAGA15000.028.0406677
TATTAGA500.007714304627.6127032
CCAATAC500.007737647327.5956133
GCAGAGT15700.026.7904479
CAGAGTA16050.025.7766210
Produced by FastQC (version 0.11.5)