FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909052
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42200.040.258091
GGTATCA18100.037.391941
TCAACGC50400.033.70114
ATCAACG50700.033.5697753
CAACGCA51350.033.2120745
TATCAAC51450.033.0141832
AACGCAG53500.031.8782186
GTGGTAT6650.031.6744211
ACGCAGA56550.030.033637
CAGAGTA56650.029.73687410
AGAGTAC56550.029.66736811
CGCAGAG59050.028.8205598
TGGTATC7250.027.6191842
GCAGAGT63400.026.8975729
AGTACGG53500.023.5506613
GAGTACG53400.023.53073512
GTACTTT19850.021.38989314
ACGGGTA4050.021.30886716
GTACGGG61700.020.19696214
TACGGGA21750.019.68057615
Produced by FastQC (version 0.11.5)