FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917039
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27290.2975882159864521No Hit
ATGTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26680.29093637238983294No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA21090.22997931385688067No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20030.21842037252505073No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT19980.21787513944336065No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18460.20130005375998183No Hit
CTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT18110.19748342218815124No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17640.19235823122026435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17620.19214013798758833No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16830.18352545529688485No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16680.1818897560518146No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16660.18167166281913855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15720.17142128088336483No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15660.17076700118533672No Hit
GTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCTCCCAGA14930.16280659819266138No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14890.16237041172730932No Hit
GTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA14250.15539142828167612No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14190.15473714858364804No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13890.15146575009350746No Hit
CTATAACATTGCTGACAATAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13710.14950291099942314No Hit
GTATAAAACACTCTGGGAGGACTTGCAGTTGATGGTGGCCCTCGCGCCCAGAGACACAGACAGGGAGGCTGGAGA13480.14699483882364872No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13360.1456862794275925No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13080.142632974170128No Hit
GTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGAGTTGGATCCG13000.14176060123942386No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12810.1396887155290015No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12800.1395796689126635No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12770.1392525290636494No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12650.1379439696675932No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12420.13543589749181878No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12040.13129212607097407No Hit
CACCTGCAGTGTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGA12020.13107403283829805No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAA11920.12998356667491787No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11830.1290021471278757No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11730.1279116809644955No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11470.12507646893970703No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11360.12387695615998884No Hit
GTCTCCAGCCTCCCTGTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCTCCCAGAGTGTTTTATA10680.1164617862490036No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10670.11635273963266557No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10310.1124270614444969No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10310.1124270614444969No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10130.11046422235041259No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10090.1100280358850605No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9860.10751996370928608No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9730.10610235769689186No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9710.10588426446421581No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9590.1045757050681596No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA9510.10370333213745545No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9460.10315809905576535No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGCCTCCCTGTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTG9210.10043193364731488No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9210.10043193364731488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.041.8010181
GTATCAA13400.040.973681
GTGGTAT1750.039.4643171
TCGTATG400.002554963634.61244240
TATCAAC16150.033.781082
TCAACGC16500.033.2719654
ATCAACG16700.032.8734973
TGGTATC2000.032.802922
CAACGCA17000.032.496485
AACGCAG17650.031.2997256
TGTTAGA5150.030.97950670
AATCTCG450.00456871130.73972936
CAGAGTA19050.028.45574410
AGAGTAC19250.028.16009911
CGCAGAG19500.027.9761378
GAGCTAT500.00770409427.6220111
GCAGAGT19850.027.4828539
ATAGACA1251.2542296E-724.8598084
ACGCAGA22000.024.795687
TAGACGG700.001327461624.6625066
Produced by FastQC (version 0.11.5)