FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917039
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27180.29638870320673383No Hit
ATGTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26380.28766497389969237No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20420.22267319056223345No Hit
CTATAACATTGCTGACAATAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC18690.20380812593575628No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18140.1978105620371653No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17800.19410297708167262No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16680.1818897560518146No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16630.1813445229701245No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA16630.1813445229701245No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT16400.1788364507943501No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16160.17621933200223763No Hit
CTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT15820.17251174704674502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14650.15975329293519686No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14630.15953519970252084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14620.1594261530861828No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14360.15659094106139435No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14270.15560952151435217No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13940.15201098317519757No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13720.14961195761576115No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13670.14906672453407105No Hit
GTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA13300.14503199972956438No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12690.13838015613294527No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12660.1380530162839312No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12420.13543589749181878No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC12320.1343454313284386No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12250.13358210501407247No Hit
GTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGAGTTGGATCCG11950.13031070652393192No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11700.12758454111548145No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11370.12398600277632685No Hit
CACCTGCAGTGTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGA11240.12256839676393261No Hit
GTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCTCCCAGA10820.11798843887773583No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10740.1171160659470317No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10660.11624369301632756No Hit
GGACTATAACATTGCTGACAATAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT10650.11613464639998954No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT10500.11449894715491926No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10290.11220896821182086No Hit
GTATAAAACACTCTGGGAGGACTTGCAGTTGATGGTGGCCCTCGCGCCCAGAGACACAGACAGGGAGGCTGGAGA10240.1116637351301308No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10210.11133659528111672No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10140.1105732689667506No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10100.11013708250139853No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC10060.10970089603604645No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9920.10817424340731419No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9770.10653854416224393No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9730.10610235769689186No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAA9510.10370333213745545No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9290.10130430657801905No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9270.10108621334534298No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9220.10054098026365292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2350.054.365771
TATCGAC200.007379686551.71248612
TGGTATC2650.048.190092
GTATCAA17200.041.7567371
GGTATCA7200.040.2845461
TATCAAC19200.037.57052
ATCAACG20850.034.5614363
TCAACGC20850.034.5501254
CAACGCA21300.033.9820145
AACGCAG21750.033.278946
CTAGTAG1600.032.34853727
TGTTAGA4550.031.08988870
GTCGGTC2150.030.4596258
AGAGTAC23300.030.3304211
CGCAGAG23950.030.0780878
TCTAGTA1750.029.57580426
CAGAGTA24300.029.21774510
GCAGAGT25150.028.6413889
ACGCAGA25700.028.1640827
GTCTATG2350.027.9175571
Produced by FastQC (version 0.11.5)