FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916750
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42700.043.066961
GGTATCA17150.041.0403371
TATCAAC50500.036.337232
TCAACGC50850.035.8826874
ATCAACG51550.035.396363
CAACGCA51650.035.3269045
AACGCAG53000.034.6873866
ACGCAGA56650.032.3281487
CGCAGAG59750.030.5931518
AGAGTAC58800.030.32490511
CAGAGTA61250.029.2245310
GCAGAGT65600.027.8649529
TTAACGG3800.025.46775435
GAGTACG49000.024.70570812
AGTACGG49500.024.5258313
GTGGTAT7000.023.165661
CGCGGTA4150.022.5725843
GAGTACT25550.022.40801612
TAACGGC4400.022.00293436
GTATTAG7300.021.2683871
Produced by FastQC (version 0.11.5)