FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916750
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44000.042.7896961
GGTATCA18200.037.3245051
TATCAAC51900.036.3115462
TCAACGC52900.035.4550554
ATCAACG53650.035.100523
CAACGCA54250.034.573665
AACGCAG55650.033.8275686
ACGCAGA59450.031.6074477
CGCAGAG62050.030.2830438
AGAGTAC61300.029.76071511
CAGAGTA64800.028.30745510
GCAGAGT67450.027.7055349
GTGGTAT8050.024.8445631
GAGTACT27850.023.60859312
AGTACGG50250.023.2931113
TAGATCG750.001897354323.19633547
AGTACTT28250.023.03180313
GAGTACG50900.022.92682312
TGGTATC8600.022.033782
GTACTTT30050.021.76789514
Produced by FastQC (version 0.11.5)