FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909609
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC18860.20734183588772762No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGTATCAGGGAGA18660.20514308895360533No Hit
GTCTCTGGTGGCTCCATCAGCGGTGGTGGTTACTACTGGACCTGGATCCG18270.20085553243206697No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16490.181286684718379No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA16040.17633950411660393No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13780.15149366376102258No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13620.14973466621372478No Hit
GTGTATTACTGTGCGAGAGATCAGGACGCTCGCTACTATGATGATAGTGCTTATCACTACGGGGGATTTGACGT13290.1461067337724231No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12520.13764155807605244No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12370.13599249787546078No Hit
CTATTACACTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGACTTACCATATCAGTAGACACGTCTAAGAA12250.13467324971498743No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGGTGGTGGTTACTACTGGA11790.12961613176650627No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11620.12774719687250236No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCACAGTGTTAGCAC11390.1252186378982618No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11280.12400932708449455No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10830.1190621464827195No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10660.11719321158871558No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT10390.11422490322765055No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10260.11279571772047109No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10000.10993734670611219No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9890.10872803589234495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9430.10367091794386381No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9380.10312123121033322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)