FastQCFastQC Report
Wed 8 May 2024
EGAF00005760656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247877
Total Bases93.2 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTA85290.6834808238311949No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT79200.6346779370082147No Hit
CTCCTAAGCTGCTCATTCACTGGGCATCTACCCGGGAATCCGGGGTCCCT78890.6321937178103291No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAAGTTGGTACCAGCAGAA78130.6261033739703512No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA72830.5836312392968218No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66970.5366714828464665No Hit
GTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCT63240.5067807163686806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC61960.49652329516450744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC59850.47961457739825314No Hit
ATATTATACTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA58810.47128042266986253No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53430.42816719917107215No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG53050.4251220272510832No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT49890.3997990186532807No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA45230.3624555945818378No Hit
ATACTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA43960.3522783094808222No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43180.3460276934345292No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41930.33601068053982885No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG40930.32799707022406854No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39380.3155759742346401No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC38300.306921275093619No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC38160.30579936964941257No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37970.30427678368941813No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT37690.3020329728010052No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35120.2814379942895013No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35040.28079690546424046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC34710.27815241406003954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34350.27526751434636587No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTGAATGAGCAGCTTAG34250.27446615331478985No Hit
GTGAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACTTA34110.2733442478705834No Hit
GCTCATTCACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCA33820.2710203008790129No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTCACTGGGCATCTACCCGGGAA32510.26052247136536694No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA31910.25571430517591076No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC31450.252028044430661No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC30800.24681919772541686No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA28480.228227621792853No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC28470.2281474856896954No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC28410.22766666907074978No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG27520.2205345558897231No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27000.21636747852552773No Hit
GTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTATTGTTGGAGCTG26930.21580652580342452No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA26060.20883468482871306No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA25390.2034655659171537No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG25320.2029046131950505No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGT25190.20186284385400166No Hit
GAGTAGTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGC24720.19809644700559428No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT24690.1978560386961215No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTC24530.19657386104559985No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC24420.1956923639108662No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT24130.19336841691929574No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24070.19288760030035013No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT23670.18968215617404602No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT23590.18904106734878517No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC22880.18335140402459538No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC22730.18214936247723132No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA22690.1818288180646009No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA22480.18014595989829127No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTGAAT22290.1786233739382968No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGC22170.17766174070040558No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22090.17702065187514474No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT22000.17629942694672632No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG21770.17445629657410144No Hit
ACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT21600.1730939828204222No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21220.17004881090043328No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21170.1696481303846453No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTGAATGAGCA20860.16716391118675958No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20150.16147424786256978No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19910.15955098138678733No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC19880.15931057307731453No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT19760.15834893983942328No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT19660.15754757880784725No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19510.1563455372604832No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19470.1560249928478528No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19310.15474281519733116No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGAC19010.15233873210260307No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT18980.15209832379313026No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18870.1512168266583966No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA18780.1504956017299782No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC18450.1478511103257773No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18310.14672920488157087No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18260.14632852436578284No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18060.1447258023026308No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG17990.14416484958052758No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17990.14416484958052758No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17920.14360389685842437No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAATATTATACTACTCCTC17580.14087926935106584No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17410.1395169555973866No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17340.1389560028752834No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA17040.13655191978055528No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC16870.13518960602687605No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC16840.13494919771740324No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA16610.13310606734477837No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC16610.13310606734477837No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16420.13158348138478393No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16390.1313430730753111No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG16240.13014103152794707No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT16230.13006089542478946No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA16090.12893898998058304No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15950.12781708453637658No Hit
GCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTCACTGGGCATCTACCC15670.12557327364796372No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA15560.12469177651323007No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15460.12389041548165403No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15130.12124592407745316No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG15100.12100551576798033No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15060.12068497135534993No Hit
TTCCCGGGTAGATGCCCAGTGAATGAGCAGCTTAGGAGGCTGTCCTGGTT14860.11908224929219786No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14700.11780007164167623No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGAGTAGTATAATATTGCTGACA14700.11780007164167623No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGAGTAGTATAATATTGCTGACAG14490.11611721347536658No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14400.11539598854694813No Hit
CAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTA14350.1149953080311601No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG14040.11251108883327443No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGG14010.11227068052380162No Hit
CTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCA14000.11219054442064402No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG13660.10946591691328553No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG13500.10818373926276387No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13490.10810360315960628No Hit
GTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCT13470.10794333095329106No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAA13430.10762278654066065No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTACT13270.10634060889013902No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT13230.1060200644775086No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCA13130.10521870344593258No Hit
TTATACTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC12980.10401666189856854No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG12930.10361598138278051No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12910.10345570917646531No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12900.1033755730733077No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12820.10273448424804688No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12780.10241393983541647No Hit
GCCTCCTAAGCTGCTCATTCACTGGGCATCTACCCGGGAATCCGGGGTCC12760.10225366762910126No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG12740.10209339542278607No Hit
GTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGA12710.10185298711331324No Hit
GGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCT12700.10177285101015565No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA12620.10113176218489482No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT12580.10081121777226441No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACAC12480.1000098567406884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)