FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320751
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.039.9121861
GTATCAA26650.037.8828281
ATCAACG30600.032.840263
AACGCAG31000.032.730966
TCAACGC31050.032.4567034
CAACGCA32000.031.4931455
TATCAAC32200.031.4388752
AGAGTAC33400.029.76340911
ACGCAGA35100.028.809697
TAACGAT605.501663E-428.67899124
CAGAGTA35350.028.4102710
CGCAGAG36050.028.2402428
ATCCCGT1856.530463E-726.77738270
CGTAACA658.756524E-426.4869652
GCAGAGT39100.026.2122949
AGTACGG31650.024.56678213
GAGTACG31950.024.44193612
GTGGTAT5450.023.3925321
TGGTATC5500.023.1640552
TCGTAAT750.001951796523.08312440
Produced by FastQC (version 0.11.5)