FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473036
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17780.3758699126493544No Hit
GTATAATAACTGGCCCTTCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT17230.3642428906045206No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14460.3056849795787213No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14050.29701756314529976No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCCGCTGATGGTGAGAGTGAACT12120.25621728578797387No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11780.2490296721602584No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10900.23042643688852435No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10660.22535282726896053No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10650.22514142686814537No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10460.2211248192526573No Hit
CCTCATGTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTT10290.21753101243879958No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATT9530.2014645819768474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9400.19871637676625037No Hit
GAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATAATAACTGG8910.1883577571263075No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8900.18814635672549235No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8840.1868779543206014No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8690.18370694830837397No Hit
TTCCTGTACAGCCTCTGGATTTAGTTTTAGTAGCTATTGGATGAGCTGGGTCCGGCAGGCTCCAGGGAAGGGG8430.17821053788717983No Hit
GAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATC8400.17757633668473435No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8230.17398252987087665No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8190.173136928267616No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8080.17081152385864926No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAG8020.16954312145375827No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATGAAGGGCCAGTTATTATACTG7980.16869751985049763No Hit
CTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCACT7850.16594931463990054No Hit
GTCCCAGACCCACTGCCACCGAACCTGGCTGGGATATCAGTGGCCCTGGT7840.1657379142390854No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT7610.1608757050203367No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7470.15791609940892448No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7470.15791609940892448No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7450.15749329860729416No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7410.15664769700403353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7340.1551678941983274No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG7340.1551678941983274No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7240.15305389019017582No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGT7170.15157408738446967No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7160.15136268698365452No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7110.15030568497957872No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7100.15009428457876356No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7070.14946008337631808No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6960.14713467896735133No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6910.14607767696327553No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTA6840.1445978741575694No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6760.14290667095104814No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6750.14269527055023298No Hit
CTCCAGCCCCTTCCCTGGAGCCTGCCGGACCCAGCTCATCCAATAGCTAC6660.14079266694289652No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6560.13867866293474493No Hit
GTGAAGGGCCGATTCACCATCTCCAGAGACAACGGCAAGAACTCACTGTA6350.13423925451762656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6300.13318225251355076No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6260.13233665091029012No Hit
ACCCTGGGGGGTCCCTGAGACTTTCCTGTACAGCCTCTGGATTTAGTTTT6170.1304340473029537No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA6130.12958844569969305No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6130.12958844569969305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6100.12895424449724757No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6080.12853144369561725No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5980.12641743968746566No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5880.12430343567931404No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA5840.1234578340760534No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA5770.12197803127034729No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACCGAACCTGGCTGGGATATCA5730.12113242966708665No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGAGACATACTA5710.12070962886545633No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT5700.12049822846464117No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT5700.12049822846464117No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA5640.11922982605975022No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5610.11859562485730472No Hit
GCCTTAGCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGC5570.11775002325404409No Hit
GTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATCCA5570.11775002325404409No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC5550.11732722245241377No Hit
GTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGG5540.11711582205159862No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5530.11690442165078346No Hit
ATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAGGTTGCTGTTAATACTCTGACTGGC5480.11584741964670767No Hit
GCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG5470.11563601924589248No Hit
CTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC5460.11542461884507732No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA5290.11183081203121961No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA5280.11161941163040445No Hit
GTGTGAGGTGCAGCTGGTGGAGTCGGGGGGAGGCTTGGTCCACCCTGGGGGGTCCCTGAGACTTTCCTGTACAGC5150.10887120641980737No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC5120.1082370052173619No Hit
TCCCTGGAGCCTGCCGGACCCAGCTCATCCAATAGCTACTAAAACTAAAT5070.10718000321328609No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT5070.10718000321328609No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT5010.10591160080839514No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG5000.10570020040757996No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC4980.10527739960594965No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG4960.10485459880431934No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4950.10464319840350418No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4920.10400899720105869No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4910.10379759680024354No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA4900.10358619639942838No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC4860.10274059479616773No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4820.1018949931929071No Hit
CGTCCATACCGTAGTAGTAGGAGTTTTGCCTAACGAGGGAGCAGGTACCA4800.10147219239127678No Hit
CATGTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCA4780.10104939158964645No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4750.10041519038720098No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCCTTCATCACCTTCGGCCA4740.10020378998638582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)