FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938046
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.038.4245261
GTATCAA16850.037.672981
ATCAACG19300.032.3509453
TCAACGC19800.031.5340044
CAACGCA20000.031.3928185
TATCAAC19950.031.2969072
AACGCAG21100.029.592736
ACGCAGA21950.028.2850827
AGAGTAC23250.026.7035511
CAGAGTA23450.026.475810
CGCAGAG24250.025.6023718
GCAGAGT26300.023.6067499
TACACCG750.001993556322.99845
AGTACGG21450.022.1895313
GAGTACG21300.022.18386712
TGGTATC3400.020.2916242
ATTAGAG2603.6379788E-1219.90143
TACGGGG11450.019.57961715
AACGGCC1952.1025699E-819.50177637
CGATATG1851.8432547E-719.297667
Produced by FastQC (version 0.11.5)