FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938046
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19450.038.4499471
GGTATCA8900.037.561021
CGTATAT400.002609711634.4632031
ATCAACG22300.033.2091333
TCAACGC22500.033.0564464
CAACGCA22450.032.9731755
TATCAAC22750.032.708892
AACGCAG23200.031.7588256
ACGCAGA25650.028.725337
AAGTCCG1305.145363E-528.33698570
AGAGTAC25800.027.62564511
CAGAGTA25950.027.59717610
CGCAGAG27150.027.011488
CGAATTA658.7426236E-426.49318915
AATCGTA1101.1680422E-625.05211618
GCAGAGT29800.024.6094579
CGAATCG1151.7216898E-623.96033716
CCGTTAA1554.1520252E-822.2236518
TAACGGC953.0330522E-421.82330136
AGTACGG23200.021.82145913
Produced by FastQC (version 0.11.5)