FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222801
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.039.530371
GGTATCA14300.037.069151
TCGTATG1500.036.95192740
CTCGTAT1550.035.72903439
CGTATGC1700.034.66669541
GTGGTAT5700.033.213591
TATCAAC40750.032.3425872
ATCAACG41350.032.0397263
TCAACGC41400.032.0010344
CAACGCA41700.031.770815
TATCTCG1750.031.5872536
TGGTATC6050.030.7143972
AACGCAG43900.030.2570366
ACGCAGA46900.028.3169867
CGCAGAG48400.027.4393928
TCTCGTA2050.026.98688738
AGAGTAC49050.026.86533711
CAGAGTA49400.026.74464410
GCAGAGT53050.025.09919
AGTACGG42150.024.24328413
Produced by FastQC (version 0.11.5)