FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966851
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20000.040.966161
GGTATCA8700.034.5706021
ATCAACG23800.034.531253
TATCAAC24100.034.265962
TCAACGC24050.034.1599244
CAACGCA24450.033.7422565
AACGCAG24850.033.199126
ACGCAGA26400.031.1191777
GTGGTAT4600.030.8129251
CGCAGAG26800.030.654718
AGAGTAC27100.029.55263511
CAGAGTA28100.028.74366410
TGGTATC4850.027.7846472
GCGATAA500.00768662427.6350125
CGAACTA2550.027.1043729
GCAGAGT31350.026.4244699
GCGAACT2750.025.1279428
CACCTAG1951.1902539E-624.99437170
AGTACGG22950.024.67209613
GAGTACG23450.024.29201512
Produced by FastQC (version 0.11.5)