FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967129
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17050.040.3280031
CGCCTAA400.00182724437.0595268
ATCAACG20150.033.36863
TCAACGC20350.033.026984
CAACGCA20350.033.0252765
AACGCAG21150.031.9406996
TATCAAC21450.031.8697282
GGTATCA8400.031.261681
GTGGTAT3000.029.94541
ACGCAGA22800.029.7803697
CGCAGAG24100.028.3169738
AGAGTAC23350.027.45946911
CAGAGTA24100.027.17285310
TATACTG1608.0035534E-1125.8486295
GCAGAGT26550.025.5741029
CGAACTA2500.023.46642529
TTTAACG2800.023.44018634
TGGTATC3550.022.368662
GTATAGG1554.0196028E-822.291861
AGCGAAC2950.022.21607427
Produced by FastQC (version 0.11.5)