FastQCFastQC Report
Wed 8 May 2024
EGAF00005760682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159492
Total Bases87 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT46740.40310756779693174No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG34780.2999589475390947No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32260.2782252917657043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32220.2778803131026346No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31690.2733093458169612No Hit
GTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA30940.2668409958844045No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT29510.25450800867966317No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28870.24898835007054815No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27080.23355055489817955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG26880.2318256615828311No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC26600.22941081094134327No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25730.22190752501957756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25300.21819900439157838No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTTTTGGATACAACTATTTGGATTGG25250.21776778106274128No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24570.21190314379055655No Hit
CTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA24370.21017825047520813No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23360.20146753923269847No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23050.19879395459390836No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA22470.19379176397939787No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22090.19051446668023583No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21980.18956577535679417No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21890.1887895733648874No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21810.188099616038748No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA21450.18499480807112081No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21430.18482231873958596No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19130.16498604561307884No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18660.16093254632200998No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18290.15774149368861537No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18210.15705153636247599No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATGGATCAGGAGCTGTG17850.1539467283948488No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17710.15273930307410488No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17670.15239432441103518No Hit
CTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG17630.15204934574796547No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCC17280.1490307824461057No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17070.14721964446498984No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16900.14575348514694364No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGT16870.1454947511496414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC16790.144804793823502No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG16070.13859517788824763No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGA15990.13790522056210824No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15660.13505914659178328No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT15590.13445543393141135No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15510.13376547660527197No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCT15470.13342049794220226No Hit
GGTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCAC15380.13264429595029548No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA15360.13247180661876062No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTG15230.13135062596378416No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15180.13091940263494703No Hit
GTCTCCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT14590.12583096735466912No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC14560.12557223335736684No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14490.12496852069699489No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTT14460.12470978669969263No Hit
GTTTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT14430.12445105270239036No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT14380.12401982937355326No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14040.1210875107374609No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13920.12005257474825182No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13800.11901763875904274No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13750.11858641543020564No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG13720.11832768143290337No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13530.11668903278332235No Hit
GTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCC13490.11634405412025266No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13440.11591283079141555No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13400.11556785212834587No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13320.11487789480220648No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13290.11461916080490421No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG13190.11375671414722999No Hit
CTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCCCT13120.11315300148685803No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG13090.11289426748955578No Hit
AGTCAGAGCCTCCTGCATAGTTTTGGATACAACTATTTGGATTGGTACCT12880.1110831295084399No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA12850.11082439551113764No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA12810.11047941684806793No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12730.10978945952192856No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAATTAT12720.10970321485616115No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12650.10909950219578919No Hit
TTATTACTGCATGCAAGCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA12630.10892701286425435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12550.10823705553811497No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12150.10478726890741807No Hit
CCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG12130.10461477957588323No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAAAACTATGC12060.10401106691551128No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11950.10306237559206963No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11910.10271739692899994No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGGAAGATGGAAGTGAACAATATTA11770.10150997160825602No Hit
GTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACT11760.1014237269424886No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG11740.10125123761095375No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11730.10116499294518634No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11630.1003025462875121No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAACC11630.1003025462875121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)