FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159492
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44840.3867210813011215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30450.26261500726180087No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27890.24053637282534074No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27730.239156458173062No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27430.2365691182000393No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC25810.2225974823457169No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25110.2165603557419974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25020.21578415375009055No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24580.21198938845632398No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24540.2116444097932543No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG24340.20991951647790583No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24020.20715968717334834No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23710.20448610253455823No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTTTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC23680.20422736853725598No Hit
CTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA23610.203623655876884No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA21350.18413236141344658No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21040.18145877677465647No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20800.17938890479623834No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20790.17930266013047091No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20510.1768878094889831No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20050.17292055486368169No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19550.16860832157531055No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18740.16162250364814937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17580.15161812241912836No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17300.14920327177764056No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16980.14644344247308305No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATGGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA16900.14575348514694364No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCC16680.14385610250006037No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16590.14307990050815356No Hit
CTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG16460.1419587198531771No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16110.1389401565513173No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15950.13756024189903854No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTGTCCCTGCCCGTCACCC15810.13635281657829462No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15800.1362665719125272No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15770.13600783791522494No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15760.13592159324945752No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15550.13411045526834164No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCA15180.13091940263494703No Hit
GGTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT15070.12997071131150537No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA15010.12945324331690083No Hit
GTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTTTTGG14990.129280753985366No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14940.1288495306565289No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14890.1284183073276918No Hit
GTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA14850.1280733286646221No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14680.1266071693465759No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14650.12634843534927365No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14550.12548598869159944No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTGTCC14510.12514101002852973No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA14460.12470978669969263No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13880.11970759608518214No Hit
GTTTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTT13770.11875890476174047No Hit
GTCTCCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT13670.11789645810406627No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13550.11686152211485719No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCCCTGA13520.11660278811755492No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13160.11349798014992772No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13130.11323924615262546No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12930.11151435283727701No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG12900.11125561883997474No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12730.10978945952192856No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12730.10978945952192856No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12600.10866827886695209No Hit
CTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCCCT12470.1075470982119756No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12450.10737460888044074No Hit
GTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCC12410.10702963021737107No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12350.10651216222276652No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12310.10616718355969683No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12300.10608093889392942No Hit
TTATTACTGCATGCAAGCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA12280.10590844956239456No Hit
AGTCAGAGCCTCCTGCATAGTTTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG12240.10556347089932488No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12110.10444229024434838No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12060.10401106691551128No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11900.10263115226323252No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11850.10219992893439542No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11850.10219992893439542No Hit
CCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG11770.10150997160825602No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11650.10047503561904697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA252.5468046E-455.28917726
ACGATAG200.007257499751.93218640
GTGGTAT2400.041.7494471
GTATCAA18300.041.3482361
GGTATCA7500.040.5401541
TGGTATC2750.037.6874162
TATCAAC20900.036.5303422
ATCAACG22250.034.3124163
TCAACGC22350.034.1588944
CAACGCA22950.033.265855
AACGCAG23050.033.121536
AGAGTAC24050.031.31071711
CAGAGTA24700.030.4867510
CGCAGAG25350.029.9775628
ACGCAGA27500.027.8850777
GTATAGA500.007679102527.641011
AATGGTA1407.5761374E-526.77324770
CAGCGCT5150.026.160524
TTCGGGG9850.026.0031270
GAGCAAA12450.025.59049270
Produced by FastQC (version 0.11.5)