FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943125
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24600.038.060381
GGTATCA11100.037.8334731
TATCAAC29750.031.9278242
ATCAACG30600.031.0392953
TCAACGC31100.030.5402724
CAACGCA31200.030.4423875
AACGCAG32650.029.3012266
ACGCGCC500.00782995727.53050611
ACGCAGA35350.027.1605787
CGCAGAG35600.026.9698438
AGAGTAC35850.026.30180711
CAGAGTA36600.025.85686310
GCAGAGT37750.025.3426519
TAATACG700.001353404324.5808094
GTTATAG1052.3203298E-522.9481741
GTGGTAT4800.022.2310411
GTATAAC1253.5713347E-622.0302431
GAGTACG25600.021.7770612
AGTACGG25650.021.7346113
ACGGGTA1605.8700607E-821.5082075
Produced by FastQC (version 0.11.5)