FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943125
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11630.12331345261762756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC350.001233266740.14652659
GTATCAA29250.039.6813661
GGTATCA12600.036.081621
TAGATCG400.002641728634.377635
TATCAAC34350.033.7575382
CAACGCA35300.033.0142145
ATCAACG35450.032.985483
TCAACGC35550.032.7837834
AACGCAG36150.032.3330426
ACGCAGA38700.030.3802347
AGAGTAC39100.029.2812411
CGCAGAG39900.029.12198
CAGAGTA39500.028.9816510
TGGTATC5500.028.1525272
GCAGAGT41450.027.9499669
GTGGTAT5800.027.9095271
TACGGGT4200.027.01815615
AGTACGG31950.025.72142813
GAGTACG31850.025.69150512
CCATTAG700.001346910424.6011051
Produced by FastQC (version 0.11.5)