FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955264
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34550.3616801219348787No Hit
CTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAAATCTGTC26100.27322289963821517No Hit
GTTTAATAGTTACCTCCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT25270.26453420206351336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24580.25731106793514674No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT22840.2390962079592657No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21100.2208813479833847No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20920.21899705212381082No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20780.21753148867747554No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18880.19764169904863993No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18550.19418715663942115No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18450.19314032560632455No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT17430.1824626490687391No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16850.17639102907677878No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG16730.17513483183706285No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16110.16864447943186386No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCCGTGCTTTAGCCTGGTATCAGCAGAAA15970.16717891598552861No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15850.1659227187458127No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15030.15733870427442048No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG14690.15377947876189202No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13640.1427877529143776No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13310.1393332105051588No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13220.13839106257537181No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT13200.13818169636875252No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12980.13587866809593996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12980.13587866809593996No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12810.13409905533967573No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAACCTCCCAAGCTCCTGAT12780.13378500602974674No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12760.13357563982312742No Hit
GGGCATTAGCCGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAACCTCCCAAGCTCCTGATGTATGATGCCTC12490.13074919603376658No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12390.12970236500066998No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12340.12917894948412167No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12310.1288649001741927No Hit
GTGTATTACTGTGCGAGACATGGGCTTCGGGATTATTATGGTTCGGGGAT12200.12771338603778642No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAAATCTGTCCCAGATCCACTGCCG12190.12760870293447674No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA12070.12635250569476084No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11900.12457289293849659No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11740.122897963285542No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11730.12279328018223236No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACT11710.12258391397561304No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11480.12017620259949081No Hit
GTCGTGTCCCTTGGCCGAAGGTGAGCGGGAGGTAACTATTAAACTGTTGA11230.1175591250167493No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11140.11661697708696235No Hit
GTCTATTATAGTGGGAGCACCTTCTACAACCCGTCCCTCAAGAGTCGAGT11040.11557014605386574No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10750.11253433605788556No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATACATCAGGAGCTTGGGAGGTTTCCCTGGTTTCTG10570.11065004019831168No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA10510.1100219415784537No Hit
GTAATACACAGCCGTGTCTGTGGCGGTCACAGAGCTCATCTTCAGGGAGA10180.1065673991692349No Hit
AGTCAGGGCATTAGCCGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAA10170.10646271606592522No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10150.1062533498593059No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9950.1041596877931127No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG9860.10321753986332574No Hit
CTCCTGATGTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT9820.1027988074500871No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCGTCC9760.10217070883022912No Hit
GTCTCCATCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9660.10112387779713251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAC200.007446778551.5944147
GTACTAA306.357475E-445.9314271
GTGGTAT2300.044.932921
GTATCAA21750.041.6550521
TCTATTA1900.039.8648342
GTCTATT1950.038.8650551
TAAGGAT5000.038.83016270
GACCGGA851.2643973E-436.84075570
TATCAAC24600.036.8079382
ATCAACG25400.035.6280983
TCAACGC25450.035.5450744
CAACGCA25950.034.8601955
AACGCAG26300.034.396286
CTATTAT2150.033.608683
CGCAGAG28100.032.3153658
AGAGTAC29150.031.15787111
CAGAGTA29400.031.00669110
TATTCTA2459.094947E-1230.67557170
GCAGAGT29650.030.626039
ACGCAGA29700.030.574477
Produced by FastQC (version 0.11.5)