FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268077
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCAGCAGTCACAGAGCTCAGGTTCAGGGAGA22100.1742796375929853No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21050.16599938331820543No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20480.1615043881404678No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15200.11986653807300346No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT15190.11978767850848174No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG14960.11797390852448235No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14140.11150742423370189No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13760.10851076078187681No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC13750.1084319012173551No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13230.10433120386222604No Hit
GTGTATTACTGTGCGAGAGAAAAAGCGGATGACTACGGTGCCTGGGGGGGCTTTGACTACTGGGGCCAGGGAACC12730.10038822563614039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.041.5485041
TAGTGAA6850.039.13496470
GGTATCA8650.038.738391
TATCAAC26700.034.2781832
ATCAACG27450.033.089993
TCAACGC27450.033.089994
CAACGCA27900.032.680075
AACGCAG28700.031.7691236
TAGACCG605.388708E-428.780745
AGAGTAC32300.028.01333811
CGCAGAG32600.027.8614838
ACGCAGA32900.027.7123977
CGTCTAA500.00759920427.7005140
CAGAGTA34400.026.3032210
GCAGAGT35250.025.6689599
CTAGTGA7000.025.1484369
TTCGGGG8800.024.79547570
AGTACGG31300.023.28205513
CAGTTCG10000.023.06687470
CGTTTAA902.1111866E-423.02459120
Produced by FastQC (version 0.11.5)