FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268077
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21000.16560508549559688No Hit
GTAATACACGGCCGTGTCCGCAGCAGTCACAGAGCTCAGGTTCAGGGAGA20290.16000605641455526No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18340.14462844133282127No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17170.13540187228378087No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT15050.11868364460517777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13790.10874733947544195No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13690.10795874383022482No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG13650.10764330557213797No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC13590.1071701481850077No Hit
GTGTATTACTGTGCGAGAGAAAAAGCGGATGACTACGGTGCCTGGGGGGGCTTTGACTACTGGGGCCAGGGAACC12970.10228085518466148No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12920.10188655736205295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27200.041.031911
TAGTGAA7750.037.6784770
GGTATCA11050.037.5238231
TATCAAC29850.037.233732
ATCAACG30800.035.9490973
TCAACGC31000.035.594674
CAACGCA31200.035.2573665
AACGCAG31600.034.9201976
ATACGAT400.002602461334.4836966
TACGTCG751.6755876E-632.184785
AGAGTAC34450.031.73225611
CGCAGAG35250.031.3031128
ACGCAGA35400.031.1716987
CAGAGTA36550.030.19092210
GCAGAGT37300.029.6751449
CTAGTGA7600.028.17515269
GTGGTAT5750.027.6425511
TGGTATC6000.027.0378172
AGTACGG32700.026.36991913
GAGTACG33200.026.0725612
Produced by FastQC (version 0.11.5)