FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662842
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35200.040.0394131
GGTATCA15900.037.7102281
ATCAACG40950.034.3238263
CAACGCA41150.034.0732885
TCAACGC41400.033.9507454
TATCAAC42750.033.120372
AACGCAG42750.032.8786166
ACGCAGA44800.031.2175127
CGCAGAG46900.029.9666088
AGAGTAC47050.029.35857811
CAGAGTA48900.028.24787510
AGCGTAG658.404763E-426.67408642
GCAGAGT53150.026.3131629
AGTACGG42850.025.0011413
GAGTACG43650.024.70076412
CGTATAC1551.551598E-924.44843
TGGTATC5350.023.8252472
GTGGTAT5850.023.5619451
GTGTATA4500.022.9728971
TATATCG750.002010269322.9666795
Produced by FastQC (version 0.11.5)