FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416488
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGGGAAAGCCCCTAAACTCCTGATCTATTCTTCATCCACTTTGCAA25310.1786813584019067No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24780.17493971004343137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18130.12799261271539186No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17630.12446275577343402No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17290.12206245305290267No Hit
GTCTCTGGTGGCGCCATCAGAAGTGGTTATTACTGGAGTTGGATCCGCCA16670.11768543044487494No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16280.11493214203014782No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15970.11274363072613393No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15580.10999034231140681No Hit
ACCATACCCTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA15520.10956675947837186No Hit
TCCCTGGTCTGTGCTGATACCAATTTAAATAGGTGTTAATGCTCTGATTTGCCCGGCAAGTGATGGTGACT15330.10822541384042786No Hit
GGGTATGGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT15050.10624869395293146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15040.10617809681409231No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14220.10038913142928142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24650.040.2029341
TATCAAC27400.036.1577532
ATCAACG28900.034.5187263
TCAACGC29200.034.4005134
CAACGCA30250.033.3205575
AACGCAG30700.032.607276
AGAGTAC35650.027.97996111
ACGCAGA36300.027.6691077
GTGGTAT4550.027.3202021
TTCGGGG8800.026.8657570
GCAGAGT38550.026.054189
CAGAGTA39200.025.79825810
TTAACGG1551.4952093E-924.52746835
CGAACTA1551.5115802E-924.50408229
GTAGTTT3352.0008883E-1124.14329370
CGCAGAG42450.023.7418168
TGTTAGA6350.023.5144970
GGTATCA17300.023.1528631
GTATTAA1352.6381713E-723.01981
AGTACGG34950.022.61502613
Produced by FastQC (version 0.11.5)